Helicase: Diferenzas entre revisións
Contido eliminado Contido engadido
Liña 120:
== Ferramentas de diagnóstico para a medida das helicases ==
=== Medida e monitorización da actividade
Various methods are used to measure helicase activity ''in vitro''. These methods range from assays that are qualitative (assays that usually entail results that do not involve values or measurements) to quantitative (assays with numerical results that can be utilized in statistical and numerical analysis). In 1982-1983, the first direct biochemical assay was developed for measuring helicase activity.<ref name="Venkatesan_1982" /><ref name="pmid6315716">{{cite journal | author = Matson SW, Tabor S, Richardson CC | title = The gene 4 protein of bacteriophage T7. Characterization of helicase activity | journal = J. Biol. Chem. | volume = 258 | issue = 22 | pages = 14017–24 |date=November 1983 | pmid = 6315716 | doi = }}</ref> This method was called a “strand displacement assay”.
|