ADN recombinante: Diferenzas entre revisións

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Miguelferig (conversa | contribucións)
Miguelferig (conversa | contribucións)
Liña 146:
: A [[hepatite B]] conrólase coa vacina da hepatite B recombinante, que contén unha forma do [[antíxeno]] de superficie do [[virus da hepatite B]] que cuxa produción se realiza en células de lévedos. O desenvolvemento da vacina de subunidades recombinante foi un importante e necesario desenvolvemento porque o virus da hepatite B, a diferena dos [[virus]] comúns como o [[virus da polio]], non pode cultivarse in vitro. [https://web.archive.org/web/20110628230253/http://www.hepb.org/hepb/vaccine_information.htm Vaccine information from Hepatitis B Foundation]
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;Diagnóstico da infección por [[VIH]]
;Diagnosis of infection with [[HIV]] : Each of the three widely used methods for [[HIV test|diagnosing HIV infection]] has been developed using recombinant DNA. The antibody test ([[ELISA]] or [[western blot]]) uses a recombinant HIV protein to test for the presence of [[antibodies]] that the body has produced in response to an HIV infection. The DNA test looks for the presence of HIV genetic material using [[reverse transcription polymerase chain reaction]] (RT-PCR). Development of the RT-PCR test was made possible by the molecular cloning and sequence analysis of HIV genomes. [https://www.cdc.gov/hiv/topics/testing/index.htm HIV testing page from US Centers for Disease Control (CDC)]
: Todos os tres grfandes métodos para [[proba do VIH|diagnosticar a infección por VIH]] desenvolvéronse utilizando ADN recombinante. A proba de [[anticorpo]]s ([[ELISA]] ou [[western blot]]) usa unha proteína do VIH recombinante para comprobar a presenza no corpo de anticorpos contra o virus. A test de ADN é outra proba na que se busca a presenza de material xenético do VIH usando a [[RT-PCR]] (reacción en cadea da polimerase con transcrición inversa). O desenvolvemento da proba de RT-PCR fíxo posible a clonación molecular e a análise de secuencias de [[xenoma]]s de VIH. [https://www.cdc.gov/hiv/topics/testing/index.htm HIV testing page from US Centers for Disease Control (CDC)]
;[[Golden rice]] : A recombinant variety of rice that has been engineered to express the enzymes responsible for [[β-carotene]] biosynthesis.<ref name="pmid|10634784" /> This variety of rice holds substantial promise for reducing the incidence of [[vitamin A deficiency]] in the world's population.<ref name="pmid|15793573">{{Cite journal
;[[Arroz dourado]]
: Unha variedade recombinante de [[arroz]] que foi preparado por enxeñaría para que expresase os encimas responsables da biosíntese do [[beta-caroteno|β-caroteno]].<ref name="pmid|10634784" /> Esta variedade de arroz é prometedor para reducir a incidencia da [[deficiencia de vitamina A]] na poboación mundial.<ref name="pmid|15793573">{{Cite journal
| last1 = Paine | first1 = J. A.
| last2 = Shipton | first2 = C. A.
Liña 167 ⟶ 169:
| year = 2005
| pmid = 15793573
}}</ref> GoldenO ricearroz isdourado notnon currentlyse inproduce use,actualmente pendingporque theestá resolutionpendente ofda regulatoryresolución andda intellectualsúa propertypropiedade intelectual e regulatoria.<ref>Deccan Herald, "
Foreign group roots for 'golden rice' in India", March 18, 2015 http://www.deccanherald.com/content/466247/foreign-group-roots-golden-rice.html</ref> issues.
;[[Glyphosate|Herbicide-resistant crops]] : Commercial varieties of important agricultural crops (including soy, maize/corn, sorghum, canola, alfalfa and cotton) have been developed that incorporate a recombinant gene that results in resistance to the herbicide [[glyphosate]] (trade name ''Roundup''), and simplifies weed control by glyphosate application.<ref name="pmid|16916934">{{Cite journal