Factor de transcrición: Diferenzas entre revisións

Os dominios de transactivación (TADs) denomínanse así pola súa composición en aminoácidos. Estes aminoácidos son esenciais para a súa actividade ou simplemente os máis abundantes no TAD. A transactivación polo sistema do factor de transcrición [[sistema GAL4/UAS|Gal4]] está mediada por aminoácidos de carácter ácido, e no sistema Gcn4 son os residuos de carácter hidrofóbico os que xogan un papel similar. Xa que logo, os dominios de transactivación en Gal4 e Gcn4 denomínanse dominios de activación ácidos ou [[hidrofóbico]]s, respectivamente.<ref name="TAD">{{Cita publicación periódica | author = Ma J, Ptashne M | title = A new class of yeast transcriptional activators | journal = Cell | volume = 51 | issue = 1 | pages = 113–9 | year = 1987 | pmid = 3115591 | doi = 10.1016/0092-8674(87)90015-8| url =}} {{Cita publicación periódica | author = Sadowski I, Ma J, Triezenberg S, Ptashne M | title = GAL4-VP16 is an unusually potent transcriptional activator | journal = Nature | volume = 335 | issue = 6190 | pages = 563–4 | year = 1988 | pmid = 3047590 | doi = 10.1038/335563a0 | url =}} {{Cita publicación periódica | author = Sullivan SM, Horn PJ, Olson VA, Koop AH, Niu W, [[Richard H. Ebright|Ebright RH]], Triezenberg SJ | title = Mutational analysis of a transcriptional activation region of the VP16 protein of herpes simplex virus | journal = Nucleic Acids Res. | volume = 26 | issue = 19 | pages = 4487–96 | year = 1998 | pmid = 9742254 | pmc = 147869 | doi = 10.1093/nar/26.19.4487| url =}}{{Cita publicación periódica | author = Gill G, Ptashne M | title = Mutants of GAL4 protein altered in an activation function | journal = Cell | volume = 51 | issue = 1 | pages = 121–6 | year = 1987 | pmid = 3115592 | doi = 10.1016/0092-8674(87)90016-X| url =}} {{Cita publicación periódica | author = Hope IA, Mahadevan S, Struhl K | title = Structural and functional characterization of the short acidic transcriptional activation region of yeast GCN4 protein | journal = Nature | volume = 333 | issue = 6174 | pages = 635–40 | year = 1988 | pmid = 3287180 | doi = 10.1038/333635a0 | url =}} {{Cita publicación periódica | author = Hope IA, Struhl K | title = Functional dissection of a eukaryotic transcriptional activator protein, GCN4 of yeast | journal = Cell | volume = 46 | issue = 6 | pages = 885–94 | year = 1986 | pmid = 3530496 | doi = 10.1016/0092-8674(86)90070-X| url =}} {{Cita publicación periódica | author = Drysdale CM, Dueñas E, Jackson BM, Reusser U, Braus GH, Hinnebusch AG | title = The transcriptional activator GCN4 contains multiple activation domains that are critically dependent on hydrophobic amino acids | journal = Mol. Cell. Biol. | volume = 15 | issue = 3 | pages = 1220–33 | year = 1995 | pmid = 7862116 | pmc = 230345 | doi =}} {{Cita publicación periódica | author = Regier JL, Shen F, Triezenberg SJ | title = Pattern of aromatic and hydrophobic amino acids critical for one of two subdomains of the VP16 transcriptional activator | journal = Proc. Natl. Acad. Sci. U.S.A. | volume = 90 | issue = 3 | pages = 883–7 | year = 1993 | pmid = 8381535 | pmc = 45774 | doi = 10.1073/pnas.90.3.883| url =}}</ref>

 

 

Os factores de transcrición de tipo 9aaTAD [[p53]], [[proteína vmw65 do virus herpes simplex|VP16]], [[MLL (xene)|MLL]], [[TCF3|E2A]], [[HSF1]], [[CEBPB|NF-IL6]], [[NFAT1]] e [[NF-κB]] interaccionan directamente cos coactivadores xerais [[TAF9]] e [[familia de coactivadores p300-CBP|CBP/p300]].<ref>{{Cita publicación periódica | author = Uesugi M, Verdine GL | title = The alpha-helical FXXΦΦ motif in p53: TAF interaction and discrimination by MDM2 | journal = Proc. Natl. Acad. Sci. U.S.A. | volume = 96 | issue = 26 | pages = 14801–6 | year = 1999 | pmid = 10611293 | pmc = 24728 | doi = 10.1073/pnas.96.26.14801}}; {{Cita publicación periódica |author=Uesugi M, Nyanguile O, Lu H, Levine AJ, Verdine GL |title=Induced alpha helix in the VP16 activation domain upon binding to a human TAF |journal=Science |volume=277 |issue=5330 |pages=1310–3 |year=1997 |pmid=9271577 |doi=10.1126/science.277.5330.1310 |url=}}; {{Cita publicación periódica |author=Choi Y, Asada S, Uesugi M |title=Divergent hTAFII31-binding motifs hidden in activation domains |journal=J. Biol. Chem. |volume=275 |issue=21 |pages=15912–6 |year=2000 |pmid=10821850 |doi= 10.1074/jbc.275.21.15912|url=}}; {{Cita publicación periódica | author = Lee CW, Arai M, Martinez-Yamout MA, Dyson HJ, Wright PE | title = Mapping the interactions of the p53 transactivation domain with the KIX domain of CBP | journal = Biochemistry | volume = 48 | issue = 10 | pages = 2115–24 | year = 2009 | pmid = 19220000 | pmc = 2765525 | doi = 10.1021/bi802055v}}; {{Cita publicación periódica |author=Goto NK, Zor T, Martinez-Yamout M, Dyson HJ, Wright PE |title=Cooperativity in transcription factor binding to the coactivator CREB-binding protein (CBP). The mixed lineage leukemia protein (MLL) activation domain binds to an allosteric site on the KIX domain |journal=J. Biol. Chem. |volume=277 |issue=45 |pages=43168–74 |year=2002 |pmid=12205094 |doi=10.1074/jbc.M207660200 |url=}}; {{Cita publicación periódica | author = Radhakrishnan I, Pérez-Alvarado GC, Parker D, Dyson HJ, Montminy MR, Wright PE | title = Solution structure of the KIX domain of CBP bound to the transactivation domain of CREB: a model for activator:coactivator interactions | journal = Cell | volume = 91 | issue = 6 | pages = 741–52 | year = 1997 | pmid = 9413984 | doi = 10.1016/S0092-8674(00)80463-8}}; {{Cita publicación periódica | author = Zor T, Mayr BM, Dyson HJ, Montminy MR, Wright PE | title = Roles of phosphorylation and helix propensity in the binding of the KIX domain of CREB-binding protein by constitutive (c-Myb) and inducible (CREB) activators | journal = J. Biol. Chem. | volume = 277 | issue = 44 | pages = 42241–8 | year = 2002 | pmid = 12196545 | doi = 10.1074/jbc.M207361200}}; {{Cita publicación periódica | author = Brüschweiler S, Schanda P, Kloiber K, Brutscher B, Kontaxis G, Konrat R, Tollinger M | title = Direct observation of the dynamic process underlying allosteric signal transmission | journal = J. Am. Chem. Soc. | volume = 131 | issue = 8 | pages = 3063–8 | year = 2009 | pmid = 19203263 | doi = 10.1021/ja809947w}}; {{Cita publicación periódica | author = Liu GH, Qu J, Shen X | title = NF-kappaB/p65 antagonizes Nrf2-ARE pathway by depriving CBP from Nrf2 and facilitating recruitment of HDAC3 to MafK | journal = Biochim. Biophys. Acta | volume = 1783 | issue = 5 | pages = 713–27 | year = 2008 | pmid = 18241676 | doi = 10.1016/j.bbamcr.2008.01.002}}; {{Cita publicación periódica | author = Bayly R, Murase T, Hyndman BD, Savage R, Nurmohamed S, Munro K, Casselman R, Smith SP, LeBrun DP | title = Critical role for a single leucine residue in leukemia induction by E2A-PBX1 | journal = Mol. Cell. Biol. | volume = 26 | issue = 17 | pages = 6442–52 | year = 2006 | pmid = 16914730 | pmc = 1592826 | doi = 10.1128/MCB.02025-05}}; {{Cita publicación periódica | author = García-Rodríguez C, Rao A | title = Nuclear factor of activated T cells (NFAT)-dependent transactivation regulated by the coactivators p300/CREB-binding protein (CBP) | journal = J. Exp. Med. | volume = 187 | issue = 12 | pages = 2031–6 | year = 1998 | pmid = 9625762 | pmc = 2212364 | doi = 10.1084/jem.187.12.2031}}; {{Cita publicación periódica | author = Mink S, Haenig B, Klempnauer KH | title = Interaction and functional collaboration of p300 and C/EBPbeta | journal = Mol. Cell. Biol. | volume = 17 | issue = 11 | pages = 6609–17 | year = 1997 | pmid = 9343424 | pmc = 232514 | doi = | url =}}; {{Cita publicación periódica | author = Piskacek S, Gregor M, Nemethova M, Grabner M, Kovarik P, Piskacek M | title = Nine-amino-acid transactivation domain: establishment and prediction utilities | journal = Genomics | volume = 89 | issue = 6 | pages = 756–68 | year = 2007 | pmid = 17467953 | doi = 10.1016/j.ygeno.2007.02.003}}</ref> p53 9aaTADs interacciona con TAF9, GCN5 e con múltiples dominios de CBP/p300 (KIX, TAZ1,TAZ2 e IBiD).<ref>{{Cita publicación periódica | author = Teufel DP, Freund SM, Bycroft M, Fersht AR | title = Four domains of p300 each bind tightly to a sequence spanning both transactivation subdomains of p53 | journal = Proc. Natl. Acad. Sci. U.S.A. | volume = 104 | issue = 17 | pages = 7009–14 | year = 2007 | pmid = 17438265 | pmc = 1855428 | doi = 10.1073/pnas.0702010104 | url =}}{{Cita publicación periódica | author = Teufel DP, Bycroft M, Fersht AR | title = Regulation by phosphorylation of the relative affinities of the N-terminal transactivation domains of p53 for p300 domains and Mdm2 | journal = Oncogene | volume = 28 | issue = 20 | pages = 2112–8 | year = 2009 | pmid = 19363523 | pmc = 2685776 | doi = 10.1038/onc.2009.71 | url =}}{{Cita publicación periódica | author = Feng H, Jenkins LM, Durell SR, Hayashi R, Mazur SJ, Cherry S, Tropea JE, Miller M, Wlodawer A, Appella E, Bai Y | title = Structural Basis for p300 Taz2/p53 TAD1 Binding and Modulation by Phosphorylation | journal = Structure | volume = 17 | issue = 2 | pages = 202–10 | year = 2009 | pmid = 19217391 | pmc = 2705179 | doi = 10.1016/j.str.2008.12.009 | url =}}{{Cita publicación periódica | author = Ferreon JC, Lee CW, Arai M, Martinez-Yamout MA, Dyson HJ, Wright PE | title = Cooperative regulation of p53 by modulation of ternary complex formation with CBP/p300 and HDM2 | journal = Proc. Natl. Acad. Sci. U.S.A. | volume = 106 | issue = 16 | pages = 6591–6 | year = 2009 | pmid = 19357310 | pmc = 2672497 | doi = 10.1073/pnas.0811023106 | url =}}{{Cita publicación periódica | author = Gamper AM, Roeder RG | title = Multivalent Binding of p53 to the STAGA Complex Mediates Coactivator Recruitment after UV Damage | journal = Mol. Cell. Biol. | volume = 28 | issue = 8 | pages = 2517–27 | year = 2008 | pmid = 18250150 | pmc = 2293101 | doi = 10.1128/MCB.01461-07 | url =}}</ref>