Electroforese en xel bidimensional: Diferenzas entre revisións

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Miguelferig (conversa | contribucións)
Miguelferig (conversa | contribucións)
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Challenges for automatic software-based analysis include incompletely separated (overlapping) spots (less-defined and/or separated), weak spots / noise (e.g., "ghost spots"), running differences between gels (e.g., protein migrates to different positions on different gels), unmatched/undetected spots, leading to [[missing values]],<ref>{{cite journal |vauthors=Pedreschi R, Hertog ML, Carpentier SC, etal |title=Treatment of missing values for multivariate statistical analysis of gel-based proteomics data |journal=Proteomics |volume=8 |issue=7 |pages=1371–83 |date=April 2008 |pmid=18383008 |doi=10.1002/pmic.200700975 }}</ref><ref>[https://web.archive.org/web/20150312131240/http://www.totallab.com/products/samespots/support/faq/missing-values.aspx What are missing values, and why are they a problem?]</ref> mismatched spots
, errors in quantification (several distinct spots may be erroneously detected as a single spot by the software and/or parts of a spot may be excluded from quantification), and differences in software algorithms and therefore analysis tendencies
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Poden utilizarse listas de picking xeradas para a [[dixextión en xel]] atomatizada de manchas de proteínas e a subseguinte identificación das proteínas por [[espectrometría de masas de proteínas|espectrometría de masas]].
 
ForPara anun overviewresumo ofdos theenfoques currentactuais approachda foranálise por software analysisde ofimaxes 2DEde gelelectroforese imagesen xel bidimensional ver see<ref>{{cite journal |vauthors=Berth M, Moser FM, Kolbe M, Bernhardt J |title=The state of the art in the analysis of two-dimensional gel electrophoresis images |journal=Appl. Microbiol. Biotechnol. |volume=76 |issue=6 |pages=1223–43 |date=October 2007 |pmid=17713763 |pmc=2279157 |doi=10.1007/s00253-007-1128-0 }}</ref> or.ou <ref>{{cite journal |vauthors=Bandow JE, Baker JD, Berth M, etal |title=Improved image analysis workflow for 2-D gels enables large-scale 2-D gel-based proteomics studies--COPD biomarker discovery study |journal=Proteomics |volume=8 |issue=15 |pages=3030–41 |date=August 2008 |pmid=18618493 |doi=10.1002/pmic.200701184 |url=http://www3.interscience.wiley.com/journal/120749796}}</ref>.
Generated picking lists can be used for the automated [[in-gel digestion]] of protein spots, and subsequent identification of the proteins by [[protein mass spectrometry|mass spectrometry]].
 
For an overview of the current approach for software analysis of 2DE gel images see<ref>{{cite journal |vauthors=Berth M, Moser FM, Kolbe M, Bernhardt J |title=The state of the art in the analysis of two-dimensional gel electrophoresis images |journal=Appl. Microbiol. Biotechnol. |volume=76 |issue=6 |pages=1223–43 |date=October 2007 |pmid=17713763 |pmc=2279157 |doi=10.1007/s00253-007-1128-0 }}</ref> or.<ref>{{cite journal |vauthors=Bandow JE, Baker JD, Berth M, etal |title=Improved image analysis workflow for 2-D gels enables large-scale 2-D gel-based proteomics studies--COPD biomarker discovery study |journal=Proteomics |volume=8 |issue=15 |pages=3030–41 |date=August 2008 |pmid=18618493 |doi=10.1002/pmic.200701184 |url=http://www3.interscience.wiley.com/journal/120749796}}</ref>
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