==Diferenzas na produción de ATP en alcalífilos==
In addition to the method of proton extrusion discussed above, it is believed that the general method of cellular respiration is different in obligate alkaliphiles as compared to neutrophiles. Generally, ATP production operates by establishing a proton gradient ( greater H+ concentration outside the membrane) and a transmembrane electrical potential ( with a positive charge outside the membrane). However, since alkaliphiles have a reversed pH gradient, it would seem that ATP production—which is based on a strong proton motive force—would be severely reduced. However, the opposite is true. It has been proposed that while the pH gradient has been reversed, the transmembrane electrical potential is greatly increased. This increase in charge causes the production of greater amounts of ATP by each translocated proton when driven through an ATPase.<ref>Higashibata, Akira, Taketomo Fujiwara, and Yoshihiro Fukumori. "Studies on the Respiratory System in Alkaliphilic Bacillus; a Proposed New Respiratory System." Extremophiles 2 (1998): 83-92. Print.</ref><ref>Hirabayashi, Toshikazu, Toshitaka Goto, Hajime Morimoto, Kazuaki Yoshimune, Hidetoshi Matsuyama, and Isao Yumoto. "Relationship between Rates of Respiratory Proton Extrusion and ATP Synthesis in Obligately Alkaliphilic Bacillus Clarkii DSM 8720T." J Bioenerg Biomembr 44 (2012): 265-72. Print.</ref> Research in this area is ongoing.
==Usos e investigacións futuras==