A aconitase é [[inhibición encimática|inhibida]] polo [[ácido fluoroacético|fluoroacetato]], polo que este composto é velenoso. O ''cluster'' de ferro-xofre é moi sensible á oxidación por [[superóxido]]s.<ref name="pmid11912933">{{cite journal |pages=9–23 |doi=10.1016/S0076-6879(02)49317-2 |chapter=Aconitase: Sensitive target and measure of superoxide |title=Superoxide Dismutase |series=Methods in Enzymology |year=2002 |last1=Gardner |first1=Paul R. |isbn=978-0-12-182252-1 |volume=349}}</ref>
==EnzymeEstrutura Structuredo encima==
AconitaseA aconitase, displayedcomo inse themostra structuresnas inestruturas theda rightdereita margindesta of this pagepáxina, hasten twodúas slightlyestruturas differentlixeiramente structuresdiferentes, dependingsegundo onse whetherestá it is activatedactivada orou inactivatedinactivada.<ref name="inactive structure">{{cite journal | author = Robbins AH, Stout CD | title = The structure of aconitase | journal = Proteins | volume = 5 | issue = 4 | pages = 289–312 | year = 1989 | pmid = 2798408 | doi = 10.1002/prot.340050406 | url = }}</ref><ref name="active structure">{{cite journal | author = Robbins AH, Stout CD | title = Structure of activated aconitase: formation of the [4Fe-4S] cluster in the crystal | journal = Proc. Natl. Acad. Sci. U.S.A. | volume = 86 | issue = 10 | pages = 3639–43 | year = 1989 | month = May | pmid = 2726740 | pmc = 287193 | doi = 10.1073/pnas.86.10.3639 }}</ref> InNa theforma inactive forminactiva, itsa structuresúa isestrutura dividedestá intodividida fouren domainscatro [[dominio proteico|dominios]].<ref name = "inactive structure"/> CountingEmpezando frompolo theextremo [[N-terminus terminal]], onlysó theos firsttres threeprimeiros ofdominios theseestán domainsimplicados areen involvedinteraccións inpróximas closeco interactions with the''cluster'' [3Fe-4S] cluster, butpero theo [[activesitio siteactivo]] consistsconsta ofde residuesresiduos frompertencentes allaos fourcatro domainsdominios, includingincluído theo largerdominio [[C- terminal]] domainmáis grnade.<ref name = "inactive structure"/> TheTamén Fe-Sse encontran no sitio activo o ''cluster'' andde aferro-xofre e un anión SO<sub>4</sub><sup>2-</sup> anion also reside in the active site.<ref name = "inactive structure"/> WhenCando these enzymeactiva iso activatedencima, itcapta gainsun anátomo additionalde ironferro atomadicional, creatingcreando a un ''cluster'' [4Fe-4S] cluster.<ref name = "active structure" /><ref name="extra structure">{{cite journal | author = Lauble H, Kennedy MC, Beinert H, Stout CD | title = Crystal structures of aconitase with isocitrate and nitroisocitrate bound | journal = Biochemistry | volume = 31 | issue = 10 | pages = 2735–48 | year = 1992 | month = March | pmid = 1547214 | doi = 10.1021/bi00125a014 }}</ref> HoweverPorén, thea structureestrutura ofdo theresto restdo ofencima thecase enzymenon is nearly unchangedcambia; theos conservedátomos atomsconservados betweenestre theestas twodúas formsformas areestán inesencialmente essentially thenas samemesmas positionsposicións, upcon todiferenzas ade difference ofsó 0.,1 angstroms[[angstrom]].<ref name = "active structure"/>
